| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159429 |
| Chromosome: | chromosome 1 |
| Location: | 3006712 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018550 | CYG69 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCTCTGCAATGCAGCGACAAGAACCCT |
| Internal bar code: | CTCGATGCACAAGTTTATCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 99.2549 |
| LEAP-Seq n confirming: | 1865 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGGAACGGATTGAAAAG |
| Suggested primer 2: | CAAGCTTACAGCCCACCTTC |