| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159440 |
| Chromosome: | chromosome 9 |
| Location: | 5642519 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401145 | (1 of 2) 3.1.4.12 - Sphingomyelin phosphodiesterase / Neutral sphingomyelinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTAGTGCAGGTGCGGGTGGAGGGATT |
| Internal bar code: | AGCGATCTGGGGGGGACTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 99.8034 |
| LEAP-Seq n confirming: | 5076 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAACTAAACCAAAGCCA |
| Suggested primer 2: | GTGTGGATGCGAGTGAGAGA |