| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159449 |
| Chromosome: | chromosome 9 |
| Location: | 4309759 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394806 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCTGCTCGAACTCGTCTCCTTCCGACT |
| Internal bar code: | TTAGGGACTAGCATATTTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 97.1671 |
| LEAP-Seq n confirming: | 686 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGCAAGTAAGTATGGGAA |
| Suggested primer 2: | GCTCTGCCTTTCTTTGATGC |