| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159466 |
| Chromosome: | chromosome 14 |
| Location: | 1270609 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616550 | CYG50 | (1 of 1) IPR000104//IPR001054//IPR006189//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // CHASE domain // Nucleotide cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTCGGCGCGCAAGCAAGGGTGCCAAGC |
| Internal bar code: | GATCCCGGGCGACAGCTTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 99.6094 |
| LEAP-Seq n confirming: | 255 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACTTGGTATCGCACCGT |
| Suggested primer 2: | GCCAAGTAGAGAGGCACTGG |