Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.159478 |
Chromosome: | chromosome_6 |
Location: | 1731189 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g261750 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGCCTTGAGGCGGGCGTGCTGCCCTCCTA |
Internal bar code: | GAGTAGGGGTCATGGTAGCGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 666 |
LEAP-Seq percent confirming: | 99.883 |
LEAP-Seq n confirming: | 5121 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGCTTCGTGGATGGATGTT |
Suggested primer 2: | GTATGAAGGTCACCGCCTGT |