| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159478 |
| Chromosome: | chromosome 8 |
| Location: | 1963814 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367750 | DIV144,GT90-12,GT90F12 | GT90 family protein 12; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGATTGGGCGCGTTTGGGGGCAGGTC |
| Internal bar code: | GCTTCATTTTCTAAATTTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 98.642 |
| LEAP-Seq n confirming: | 1816 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCAAGAAGCTTCCCAGCA |
| Suggested primer 2: | CCTACCGCTATCTCAGCCAG |