Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.159504 |
Chromosome: | chromosome_2 |
Location: | 5090054 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g104900 | FAP204 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACCCGCAATCACGCACCTCCTTGTTGAGG |
Internal bar code: | GTGAGTTACAAGGAAGCAAAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 745 |
LEAP-Seq percent confirming: | 99.9057 |
LEAP-Seq n confirming: | 1059 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCCTTCTCGTCTGCAGGTC |
Suggested primer 2: | TGAAAGCTTGGTGCTGAATG |