| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159505 |
| Chromosome: | chromosome 9 |
| Location: | 6125745 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405150 | PUS1 | RNA pseudouridine synthase; (1 of 2) PTHR11142//PTHR11142:SF0 - PSEUDOURIDYLATE SYNTHASE // TRNA PSEUDOURIDINE SYNTHASE-LIKE 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTCCTGCCGCCGGACGTGAGGGTGCTG |
| Internal bar code: | CGGGGCGACGTAGAATGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 99.7435 |
| LEAP-Seq n confirming: | 5832 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAATGATCCGTGCTCCGT |
| Suggested primer 2: | CTACCTTGGTCTCGCCTCAG |