| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159550 |
| Chromosome: | chromosome 2 |
| Location: | 4527275 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100633 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCTGCCAGCAGGGTGGTCAAGCGGGAGC |
| Internal bar code: | GGGGGATACGTGGCAAAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 64.0 |
| LEAP-Seq n confirming: | 208 |
| LEAP-Seq n nonconfirming: | 117 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATGCATAACGCAGCCTA |
| Suggested primer 2: | TGACGTTAAGCAGGATGCAG |