| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159580 |
| Chromosome: | chromosome 1 |
| Location: | 2588037 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015050 | AGK1 | (1 of 1) IPR006569//IPR017956//IPR020478 - CID domain // AT hook, DNA-binding motif // AT hook-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTACCGCCATTTGCAAGTCTCGTTGGACT |
| Internal bar code: | GCTTCCGCAACTACTCAATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCTAACGTGCATCGAGA |
| Suggested primer 2: | GGTGTCTGGTTTGAGGTCGT |