Insertion junction: LMJ.RY0402.159595_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.159595
Chromosome:chromosome 10
Location:1505924
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g428750 (1 of 1) K14792 - rRNA biogenesis protein RRP5 (RRP5, PDCD11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATGGCACGCCGCTTCACATGCTCCACG
Internal bar code:GCGCACGTTTGGATGCGGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:990
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTACTGCCAACTCCCCT
Suggested primer 2:CAACTGCACACAGGCATCTT