| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159597 |
| Chromosome: | chromosome 2 |
| Location: | 3066394 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095048 | (1 of 726) IPR011009 - Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCGCTGGCATTGGCGTCGCTGACCTTT |
| Internal bar code: | GAGACGGATTAGAAAGGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 89.0278 |
| LEAP-Seq n confirming: | 641 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGTGCATAGTCATGGTG |
| Suggested primer 2: | CACAGCTTCTCTGACCCCTC |