| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159601 |
| Chromosome: | chromosome_16 |
| Location: | 1629749 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g654300 | RSP23 | Radial spoke protein 23 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGCCCGATGCCGTGCGGGCCGGCAAGGCG |
| Internal bar code: | CGGCCCGCTTTAGGCTAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 99.6964 |
| LEAP-Seq n confirming: | 985 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTATGCAGCTCTGGGTCA |
| Suggested primer 2: | GCGTACCACTTAGGCTGCTC |