| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159662 |
| Chromosome: | chromosome 9 |
| Location: | 4463289 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395695 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCATCGCGTGCAACTACTGCCGTCGTC |
| Internal bar code: | GGGCTCCAACGCCCGTATTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 82.9768 |
| LEAP-Seq n confirming: | 6439 |
| LEAP-Seq n nonconfirming: | 1321 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTGAGCCTGCTCATTGG |
| Suggested primer 2: | AGACGTCGGCTAACGATTTG |