Insertion junction: LMJ.RY0402.159662_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.159662
Chromosome:chromosome 9
Location:4463293
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g395695 (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGGACCCTCGTCCGCGTCTGCCATGGCC
Internal bar code:GGACGCGTGTAGTATGTGGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:526
LEAP-Seq percent confirming:99.68
LEAP-Seq n confirming:13705
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACTTGAGCCTGCTCATTGG
Suggested primer 2:AGACGTCGGCTAACGATTTG