Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.159699 |
Chromosome: | chromosome_2 |
Location: | 5990394 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g112200 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCCAGGGCCCACTGGTGCTTCACCGCTGC |
Internal bar code: | AGCCGCAGACCGCACTTCCCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 668 |
LEAP-Seq percent confirming: | 77.5811 |
LEAP-Seq n confirming: | 789 |
LEAP-Seq n nonconfirming: | 228 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCTGCAGTGCTTGACATTA |
Suggested primer 2: | CCTGTTCTGTTCGTGGACCT |