| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159703 |
| Chromosome: | chromosome 4 |
| Location: | 3139992 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226001 | (1 of 1) IPR000104//IPR015943 - Antifreeze protein, type I // WD40/YVTN repeat-like-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGAGTGATGCCAACAGGCAACTTGGGG |
| Internal bar code: | CATTAAAGTTGCAGGCTTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.8041 |
| LEAP-Seq n confirming: | 1019 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGTCTGAACGGCGAAAG |
| Suggested primer 2: | ACTACACCACATGCCAACCA |