| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159704 |
| Chromosome: | chromosome 13 |
| Location: | 763621 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566750 | (1 of 2) PTHR31385//PTHR31385:SF1 - FAMILY NOT NAMED // EXPRESSED PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACCGTACGTTCGCAAGGTCCTGTACAC |
| Internal bar code: | AAGGTAGAGTGTGCGGCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 99.5446 |
| LEAP-Seq n confirming: | 1093 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGAGGGAGCTGAGAAG |
| Suggested primer 2: | GGCAAGCAAAAAGAAAGTGC |