| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159718 |
| Chromosome: | chromosome 8 |
| Location: | 4164036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380000 | UMM7 | UbiE/COQ5 methyltransferase family protein; (1 of 1) PTHR10108//PTHR10108:SF894//PTHR23150//PTHR23150:SF24 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED // SULFATASE MODIFYING FACTOR 1, 2 // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAAGCCAGCCCGACAAAAAGGCAACAA |
| Internal bar code: | GATTACGCGCGTGCGGCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 99.4179 |
| LEAP-Seq n confirming: | 3928 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAGCAAGACCTTCATCG |
| Suggested primer 2: | GTATGGCTTGGATGCTGGAT |