| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159719 |
| Chromosome: | chromosome 9 |
| Location: | 402818 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404800 | (1 of 20) IPR008972 - Cupredoxin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTAGCGGTTGTGGCGTCAATGGCGGCAG |
| Internal bar code: | GGTGTATGAAACACACCCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 99.5305 |
| LEAP-Seq n confirming: | 1060 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTACTCGATGCAGGTGAT |
| Suggested primer 2: | GTCTTCCACCTGCCGTATGT |