| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159740 |
| Chromosome: | chromosome 3 |
| Location: | 5538612 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186200 | CDS1,PCT1 | Phosphatidate cytidylyltransferase; (1 of 1) PTHR13773:SF8 - PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCAAACCTGGCTCAGCTACCAGCCAGT |
| Internal bar code: | AAGATACGCGGGGAAACACCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1132 |
| LEAP-Seq percent confirming: | 98.0623 |
| LEAP-Seq n confirming: | 2328 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACAAGTTGGGAAGGTGCC |
| Suggested primer 2: | TGGTCCTACATCTCCAAGCC |