| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159741 |
| Chromosome: | chromosome_9 |
| Location: | 1708211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g396800 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACTCGACGGACGGCTCTAGTCGATCACAA |
| Internal bar code: | TCACGGTGACCGCCCTTTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 99.4949 |
| LEAP-Seq n confirming: | 3349 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCCAGGCTCTAACAATG |
| Suggested primer 2: | CAGAATGGAACGTGGTTGTG |