| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159747 |
| Chromosome: | chromosome 2 |
| Location: | 600777 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077800 | CSE13,FAP310 | Flagellar Associated Protein 310 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCTTGTGTGTAAGGTTGAGACTGTGG |
| Internal bar code: | ACTTGCGGGTAATCCGGTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 48.2998 |
| LEAP-Seq n confirming: | 4460 |
| LEAP-Seq n nonconfirming: | 4774 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGATGTGCGCTAGTTTG |
| Suggested primer 2: | GAATCGTGGATTATGGGGTG |