| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159827 |
| Chromosome: | chromosome 14 |
| Location: | 3264132 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629500 | (1 of 1) PF06244 - Protein of unknown function (DUF1014) (DUF1014) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCAGAAGGCGCCCGACAACCCGCTCAA |
| Internal bar code: | GTTAGTAAATTGCTTTGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1099 |
| LEAP-Seq percent confirming: | 65.5172 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCTATACGCCAGCTAC |
| Suggested primer 2: | TTTACACACACCTCCCGACA |