| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159862 |
| Chromosome: | chromosome 8 |
| Location: | 3254459 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373688 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTTGTAGATACCAGCCCAAACTAAATC |
| Internal bar code: | GGTGATGGACATATCCGGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 99.2514 |
| LEAP-Seq n confirming: | 1591 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTAACCCCCTCCTCACC |
| Suggested primer 2: | AAGGAGAAGTGCAAGGACGA |