| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.159873 |
| Chromosome: | chromosome 17 |
| Location: | 3503822 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCTGAACCCAGGGCCATGCGTGCGAGC |
| Internal bar code: | CGGGTATTTGGCCCGTACGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.2228 |
| LEAP-Seq n confirming: | 3830 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACAAGGACATTGTGGTG |
| Suggested primer 2: | TTTCCCCTCACACACAAACA |