| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159888 |
| Chromosome: | chromosome 7 |
| Location: | 6256330 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g356600 | (1 of 1) PF08007 - Cupin superfamily protein (Cupin_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGGGGGCCGCCCGGGGGCTCCCTCCA |
| Internal bar code: | AGCTGTTCCCTAGACGGGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 97.3753 |
| LEAP-Seq n confirming: | 371 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACGGCGAGGGTATCGTA |
| Suggested primer 2: | CTCACTGCATGAGGAAGTCG |