Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.159901 |
Chromosome: | chromosome_1 |
Location: | 6929149 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g049950 | VLE1,SPO | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCGCACCTCTGCTTGTTTTGGAGGACAGC |
Internal bar code: | GTGAGGTGGGTGCATGGGGTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 824 |
LEAP-Seq percent confirming: | 83.7086 |
LEAP-Seq n confirming: | 632 |
LEAP-Seq n nonconfirming: | 123 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACATCTGAATATGGGGCAGC |
Suggested primer 2: | CCTGTTCTGTTTTGGCCAGT |