| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.159901 |
| Chromosome: | chromosome_1 |
| Location: | 6929190 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g049950 | VLE1,SPO | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTAAAATAATGTAGGCTAGGGCCCGCGGCC |
| Internal bar code: | GGGTCCTGAGATGAAATGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1110 |
| LEAP-Seq percent confirming: | 99.2458 |
| LEAP-Seq n confirming: | 1316 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTCACTGATTTTGGAGT |
| Suggested primer 2: | ACATCTGAATATGGGGCAGC |