| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.159946 |
| Chromosome: | chromosome 3 |
| Location: | 825625 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146447 | (1 of 1) PTHR23244//PTHR23244:SF253 - KELCH REPEAT DOMAIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACTGTGTGTTGCACCGCTCACTCTCCC |
| Internal bar code: | AGCCGCTCGGGCTACATTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 35 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCGGCTTGGTACTGTTTG |
| Suggested primer 2: | AAACATGCTCCCTCCTGTTG |