| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.159960 |
| Chromosome: | chromosome 9 |
| Location: | 5541089 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400553 | TOR1 | (1 of 1) K07203 - FKBP12-rapamycin complex-associated protein (FRAP); Target of rapamycin 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACAGGCCACAGCCCAGGTCCGAGGTTT |
| Internal bar code: | TAGGGTAGCAGGTAGTGGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 747 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGATCTGCGGACTGACAC |
| Suggested primer 2: | GGTCAACCACGAGAACCTGT |