Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.159995 |
Chromosome: | chromosome 8 |
Location: | 3581386 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g375800 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCCTCTTGTTACAGTGTCATGCCAATG |
Internal bar code: | CCCTACATCTTGGTTTCGCATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 249 |
LEAP-Seq percent confirming: | 99.6868 |
LEAP-Seq n confirming: | 3183 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAATCAAGACAAGCAAGCA |
Suggested primer 2: | CAGGACTTGTAGGGCACCAT |