| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160042 |
| Chromosome: | chromosome 15 |
| Location: | 180443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCTCGAACAGCATGCAAACCAGTGCCC |
| Internal bar code: | AGGGGAATATGGTCCAAGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 486 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACTATTCGTGTCCCCT |
| Suggested primer 2: | GAGACGGGCACGTAACATTT |