| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160074 |
| Chromosome: | chromosome 12 |
| Location: | 7626997 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555001 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACCTCGGCTCTAGAGGTTGCGCCTGTG |
| Internal bar code: | ACCGTTCTCCGGGCATCGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 98.5171 |
| LEAP-Seq n confirming: | 2126 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATCCATCCCCGTCATCAG |
| Suggested primer 2: | TGGAGGTGAGCAACATGGTA |