| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160079 |
| Chromosome: | chromosome 12 |
| Location: | 2940305 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501600 | BLZ8 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2); bZIP transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTATGAAGGTGGTAGTGGGAACGGCGG |
| Internal bar code: | GTATGTATGGATGTGTCGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 99.876 |
| LEAP-Seq n confirming: | 1611 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCCCCCTTAGATAGGC |
| Suggested primer 2: | CGAGGGCGAGACTAGACAAC |