| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160087 |
| Chromosome: | chromosome 10 |
| Location: | 2195215 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433800 | CSE21 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGATCCTTGAAGCATGCGGCGCGCGTCG |
| Internal bar code: | GCGCTGAAGGTTCGACCCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 98.9372 |
| LEAP-Seq n confirming: | 1024 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATTCTGGAGCTGCTTGA |
| Suggested primer 2: | TGTCCTCAACAGCACAGAGG |