Insertion junction: LMJ.RY0402.160111_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.160111
Chromosome:chromosome 4
Location:1762195
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g212200 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCATGTTGTCCCTTGGCCCTTGCCCAT
Internal bar code:TTTAATCGGGTACACAAGGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:967
LEAP-Seq percent confirming:99.778
LEAP-Seq n confirming:899
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAACTCAAACCCAAGACC
Suggested primer 2:CGACGTGGAGACTAGAAGGC