| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160113 |
| Chromosome: | chromosome 1 |
| Location: | 2453638 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013800 | SXD1,TCY1 | (1 of 1) PF14392 - Zinc knuckle (zf-CCHC_4); Zinc finger containing protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAGATGACCAAGCTGTTGCGCAACCCG |
| Internal bar code: | CGTGAGCTTGCTCGTTTGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 92.3757 |
| LEAP-Seq n confirming: | 51105 |
| LEAP-Seq n nonconfirming: | 4218 |
| LEAP-Seq n unique pos: | 107 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATGTCCACAGACAGCGA |
| Suggested primer 2: | CTGAAGTAGTCGCCTTTGCC |