| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160127 |
| Chromosome: | chromosome 7 |
| Location: | 649982 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317100 | POB18 | Proteome of basal body 18 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTGAGCAGGAGGCTGCTGAACTCGGCG |
| Internal bar code: | CGAGCCAGACGCCCTGGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 184 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATAGCCGGTACTTCAAGC |
| Suggested primer 2: | CTCGTTGCCTGTGTGAGTGT |