| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160137 |
| Chromosome: | chromosome_5 |
| Location: | 1321195 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g244800 | NUOAF3 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGGCGGCGATGGAGGTGAAGGGCAGGCGG |
| Internal bar code: | GACTGAGGGACGCTGTGCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 99.4943 |
| LEAP-Seq n confirming: | 787 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGTTACATTCATCATGC |
| Suggested primer 2: | CTGAAGATGAAGGGGGTGAA |