| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160167 |
| Chromosome: | chromosome 10 |
| Location: | 2192438 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433750 | PAP1 | Class-I Nucleotidyl-transferase 1, nuclear; (1 of 1) K14376 - poly(A) polymerase (PAP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGGCAATTCTCCAACATACCCGCATC |
| Internal bar code: | GGAAGCGTTGCTCGCCTGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 99.0482 |
| LEAP-Seq n confirming: | 7597 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGATGACGCTGGAGAAG |
| Suggested primer 2: | GGTAGTTCACTCCGATCCCA |