| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160168 |
| Chromosome: | chromosome_9 |
| Location: | 2271488 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392500 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAATCCAAGGGCGGCGTAATTTATGACCG |
| Internal bar code: | CTTCACGTGATTTACAGCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 99.7619 |
| LEAP-Seq n confirming: | 838 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAATAGAGGAGGCGGGAG |
| Suggested primer 2: | CCCACATTCTATGATCGCCT |