| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160179 |
| Chromosome: | chromosome_1 |
| Location: | 4327975 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029350 | C1d-87,FAP297 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTTTCGCCGTGGTTTATGATTCTTTCTCT |
| Internal bar code: | ACACTGTTCATGCGTGCTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.488 |
| LEAP-Seq n confirming: | 13019 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTTTGCTTGTGGTGTTGG |
| Suggested primer 2: | ACTCCCTATCCAACACGCAC |