| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160209 |
| Chromosome: | chromosome 1 |
| Location: | 243500 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001550 | CIF3,TIF3A,TIF3 | (1 of 1) K02520 - translation initiation factor IF-3 (infC, MTIF3); Chloroplast translation initiation factor 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGGGCGCAAGAGCCTCACCCGCCTCT |
| Internal bar code: | CCGCGTCGATGTTACGGGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.6 |
| LEAP-Seq n confirming: | 996 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACAGCTACACCCCAATCT |
| Suggested primer 2: | CGGTGTGGAAAGTGTTGATG |