| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160250 |
| Chromosome: | chromosome 16 |
| Location: | 6143738 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675600 | (1 of 5) IPR000104//IPR001214 - Antifreeze protein, type I // SET domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCGCCAGCGCACGCCACCAGCCCTCC |
| Internal bar code: | TTGGGTTCGGCGGGCGCGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 88.7324 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCATTCCTTTGCCGTTTA |
| Suggested primer 2: | GGTTGTACGGCTTTTCTGGA |