| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160258 |
| Chromosome: | chromosome 8 |
| Location: | 2514217 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372000 | BAF3,CGLD6,CGLD11 | chloroplast ATPase CF1 assembly factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGGAGGGACGCAAGGGGGAGGGCGGA |
| Internal bar code: | ACCGTGTGAGGGTTGATTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 99.6923 |
| LEAP-Seq n confirming: | 324 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCTAGTGAAGCACGAGG |
| Suggested primer 2: | CTGCCTCCATACACATGCAC |