| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160261 |
| Chromosome: | chromosome 16 |
| Location: | 5608724 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679850 | CYG35 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCCCCGTGTGCCTCCTCGTGTACAAAT |
| Internal bar code: | ATGACGAGGAGAAGGTGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 98.8528 |
| LEAP-Seq n confirming: | 517 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGAGAGTGGAAGCCTGG |
| Suggested primer 2: | TGCTTTGCTAAACATCGTCG |