| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160281 |
| Chromosome: | chromosome 6 |
| Location: | 8590132 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308750 | (1 of 4) PF03006 - Haemolysin-III related (HlyIII) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCTGGTTGCTATGGAGCTGTGATCTCC |
| Internal bar code: | GGGGCCACCGCCCCCTGACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 90 |
| LEAP-Seq percent confirming: | 99.7155 |
| LEAP-Seq n confirming: | 701 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGCCCAGTCCATACTAA |
| Suggested primer 2: | GCAGAGGAAGTAGAGCCCCT |