| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160300 |
| Chromosome: | chromosome_5 |
| Location: | 1177161 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g245500 | FAP175,ANK24 | Flagellar Associated Protein with ankyrin repeats | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGTCGACCATGTGGGCGAACCTACTCCTG |
| Internal bar code: | ACTGGGTCGTGATCCAAGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 869 |
| LEAP-Seq percent confirming: | 99.6774 |
| LEAP-Seq n confirming: | 618 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGCCGAGTCTTGGAAGT |
| Suggested primer 2: | TACACGCTGGACTTGAGACG |