Insertion junction: LMJ.RY0402.160303_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.160303
Chromosome:chromosome 16
Location:3547457
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g679164 (1 of 1) K15433 - RLL motif containing protein 1 (CGI99, CLE7, RLLM1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGACCTTGCACCCCAAGGACGGAGTCCAC
Internal bar code:CATCGCCGCAAAGCGTTTGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:721
LEAP-Seq percent confirming:99.0596
LEAP-Seq n confirming:948
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGAGTGTAGACGTTTGGG
Suggested primer 2:CGACTTCATGGCAGCAGTAA